Spore Coat Proteins of D. discoideum |

Spore Coat Proteins of D. discoideum

AlexanderLab

The Division of Biological Sciences .:. The University of Missouri

Spore Coat Proteins of D. discoideum

Spore coat proteins from
D. discoideum

Compiled by Supriya Srinivasan

Protein
Gene Identification Antibody Characteristics References
SP200/p200 [35S]Met labeling
1D and 2D PAGE silver staining
MUD143 Immunoprecipitates with PsB from spores 1-3
SP170 [35S]Met labeling
2D PAGE
Acidic glycoprotein: pI 4.5-4.8 4
p102/p104 [35S]Met labeling Immunoprecipitates with PsB from spores 3, 5
SP96/p112 CotA [35S]Met labeling [32P]Pi labeling
EM
IF
MUD141
MUD62
mAb 83.5
On outer spore coat layer and interspore matrix
Part of the PsB multiprotein complex
Modified by O-linked fucose and phosphodiester-linked N acetylglucosamine
pI 4.5-4.8
Essential for spore coat viability
5-12
SP97 [35S]Met labeling
2D PAGE
Acidic glycoprotein: pI 4.5-4.8
Migrates with SP90 on 1D PAGE at 94kD
4
SP90 [35S]Met labeling
2D PAGE
Acidic glycoprotein: pI 4.5-4.8.
Migrates with SP97 on 1D PAGE at 94 kD
4
SP87 Western blots
EM
IF
PL3
Anti-PL3 Cross linked to the middle spore coat layer 13
PsB/SP85 pspB [35S]Met labeling
EM
IF
MUD102
MUD50
mAb 16.1
On inner spore coat layer
Part of the PsB multiprotein complex
Has in vitro cellulose binding activity
3, 5, 7, 9, 14
SP82 [35S]Met labeling
2D PAGE
Acidic glycoprotein: pI 4.5-4.8 4
SP80 mAb 5.1 15
SP76 [35S]Met labeling
2D PAGE
Acidic glycoprotein: pI 5.9 4
SP75 DP87 [35S]Met labeling [32P]Pi labeling
EM
MUD62
mAb 83.5
On outer spore coat layer
Modified by N- glycosylation and O fucose
Crosslinked into coat
Immunoprecipitates with PsB from spores
5-7, 16, 17
SP72 [35S]Met labeling
2D PAGE
Acidic protein: pI 4.5-4.8 4
SP70/p78 CotB [35S]Met labeling
EM
2H3 On outer spore coat layer
Modified by O-fucose
Part of the PsB multiprotein complex
Essential for spore coat viability
3, 6, 10, 17, 18
SP68 Coomassie Blue
[35S]Met labeling
1D PAGE
Minor coat protein 1, 19
p63 [35S]Met labeling
1D PAGE
Immunoprecipitates with PsB from spores 3, 5
SP60/p58 CotC Coomassie Blue
[35S]Met labeling
2D PAGE
On inner spore coat layer
Part of the PsB multiprotein complex
1, 3, 19
SP55 [35S]Met labeling
2D PAGE
Acidic protein: pI 5.7 4
p45 [35S]Met labeling
1D PAGE
Immunoprecipitates with PsB from spores 3
SP39 [35S]Met labeling
2D PAGE
Minor coat protein 4
SP33 [35S]Met labeling
2D PAGE
Basic protein: pI 6.6-7.5 4
SpiA Dd31 Western blots
SpiA
anti SpiA On inner spore coat layer
Essential for spore coat viability
20, 21
SP3 [35S]Met labeling
2D PAGE
Minor coat protein 4

REFERENCES

1 Orlowski, M. and Loomis, W.F. (1979) Plasma membrane
proteins of Dictyostelium: the spore coat proteins.
Dev. Biol. 71, 297-307
2 Devine, K.M. et al. (1983) Spore coat proteins of
Dictyostelium discoideum are packaged in prespore
vesicles. Dev. Biol. 99, 437-446
3 Watson, N. et al. (1993) A developmentally regulated
glycoprotein complex from Dictyostelium discoideum. J.
Biol. Chem.
268, 22634-22641

4 Wilkinson, D. et al. (1983) Synthesis of spore
proteins during development of Dictyostelium discoideum.
Biochem. J. 216, 567-574
5 Watson, N. et al. (1994) The PsB glycoprotein complex
is secreted as a preassembled precursor of the spore coat in
Dictyostelium discoideum. J. Cell Sci. 107,
2567- 2579

6 Champion, A. et al. (1991) Immunodominant
carbohydrate determinants in the multicellular stages of
Dictyostelium discoideum. J. Gen. Microbiol. 137,
2431-2438

7 West, C.M. and Erdos, G.W. (1990) Formation of the
Dictyostelium
spore coat. Dev. Genet. 11,
492-506

8 Fosnaugh, K.L. et al. (1994) Structural roles of the
spore coat proteins in Dictyosteium discoideum. Dev.
Biol.
166, 823-825

9 McGuire, V. and Alexander, S. (1996) PsB multiprotein
complex of Dictyostelium discoideum: Demonstration of
cellulose binding activity and order of protein subunit
assembly. J. Biol. Chem. 271, 14596-14603
10 Srinivasan, S. et al. (1999) The cellulose binding
activity of the PsB mutiprotein complex is required for proper
assembly of the spore coat of Dictyostelium discoideum. In
preparation

11 Tasaka, M. et al. (1990) Isolation and
characterization of spore coat protein (sp96) gene of
Dictyostelium discoideum. Cell Differ. Dev. 31,
1-9

12 Srinivasan, S. et al. (1995) unpublished.
13 Yoder, B.K. et al. (1994) Identification of a new
spore coat protein gene in the cellular slime mold Dictyostelium
discoideum.
Dev. Biol. 163, 49-65

14 Zhang, Y.Y. et al. (1998) Two proteins of the
Dictyostelium spore coat bind to cellulose in vitro.
Biochemistry 37, 10766-10779
15 West, C.M. and Erdos, G.W. (1988) The expression of
glycoproteins in the extracellular matrix of the cellular slime mold
Dictyostelium discoideum. Cell. Differ. 23,
1-16

16 West, C.M. et al. (1996) SP75 is encoded by the DP87
gene and belongs to a family of modular Dictyostelium
discoideum
outer layer spore coat proteins.

Microbiology 142, 2227-2243
17 Gonzalez-Yanes, B. et al. (1989) The spore coat of a
fucosylation mutant in Dictyostelium discoideum. Dev.
Biol.
133, 576 587

18 Gomer, R. (1987) A strategy to study development and
pattern formation: use of
antibodies against products of cloned genes. Meth. Cell
Bio.
l 1987, 471-487

19 Devine, K.M. et al. (1982) Differential synthesis of
spore coat proteins in prespore and prestalk cells of
Dictyostelium. Proc. Natl. Acad. Sci. USA 79,
7361-7365

20 Richardson, D.L. and Loomis, W.F. (1992) Disruption of the
sporulation-specific gene spiA in Dictyostelium
discoideum
leads to spore instability. Genes Dev.

6, 1058-1070
21 Richardson, D.L. et al. (1991) A prespore gene,
Dd31, expressed during culmination of Dictyostelium
discoideum.
Dev. Biol. 144, 269-280